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piezo-type mechanosensitive ion channel component 1 (piezo1) (ProMIS Neurosciences)

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ProMIS Neurosciences piezo-type mechanosensitive ion channel component 1 (piezo1)
Piezo Type Mechanosensitive Ion Channel Component 1 (Piezo1), supplied by ProMIS Neurosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piezo-type+mechanosensitive+ion+channel+component+1+%28piezo1%29/pm38123150-1239-10-18?v=ProMIS+Neurosciences
Average 90 stars, based on 1 article reviews

piezo-type mechanosensitive ion channel component 1 (piezo1) - by Bioz Stars, 2026-07

90/100 stars

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(A) Representative tracing of contractile response to cumulative additions of the <t>Piezo1</t> agonist Yoda1 to thoracic aorta ring with PVAT. Piezo1 agonists did not cause a concentration dependent contraction. Quantification of multiple experiments using Yoda1 (B) and Jedi2 (C) as agonists (grey) vs Vehicle (white, same vehicle for B and C). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

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Image Search Results

(A) Representative tracing of contractile response to cumulative additions of the Piezo1 agonist Yoda1 to thoracic aorta ring with PVAT. Piezo1 agonists did not cause a concentration dependent contraction. Quantification of multiple experiments using Yoda1 (B) and Jedi2 (C) as agonists (grey) vs Vehicle (white, same vehicle for B and C). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

Journal: Pharmacological research

Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

doi: 10.1016/j.phrs.2021.105995

Figure Lengend Snippet: (A) Representative tracing of contractile response to cumulative additions of the Piezo1 agonist Yoda1 to thoracic aorta ring with PVAT. Piezo1 agonists did not cause a concentration dependent contraction. Quantification of multiple experiments using Yoda1 (B) and Jedi2 (C) as agonists (grey) vs Vehicle (white, same vehicle for B and C). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

Techniques: Concentration Assay

(A) Representative tracing of relaxant response to cumulative additions of the Piezo1 agonist Yoda1 in half-maximally PE contracted thoracic aorta ring with PVAT. Neither the Piezo1 agonist Yoda1 (B) nor Jedi2 (C) (grey) caused concentration dependent relaxation vs Vehicle (black). (A) Arrows indicate each cumulative step. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

Journal: Pharmacological research

Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

doi: 10.1016/j.phrs.2021.105995

Figure Lengend Snippet: (A) Representative tracing of relaxant response to cumulative additions of the Piezo1 agonist Yoda1 in half-maximally PE contracted thoracic aorta ring with PVAT. Neither the Piezo1 agonist Yoda1 (B) nor Jedi2 (C) (grey) caused concentration dependent relaxation vs Vehicle (black). (A) Arrows indicate each cumulative step. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

Techniques: Concentration Assay

Piezo1 mRNA is present in vessels and PVAT of the male Sprague Dawley rat aorta (A) and mesenteric vessel (B) as well as in the adipocytes and SVF of the mesenteric PVAT(C). Piezo1, Piezo2, TRPV4, TMEM16, and Panx1 mRNA were measured using RT-PCR and beta actin as a calibrator control. Bars are mean+SEM with scattered individual points for an N = 4–5. Stat test was a one-way ANOVA, *p < 0.05.

Journal: Pharmacological research

Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

doi: 10.1016/j.phrs.2021.105995

Figure Lengend Snippet: Piezo1 mRNA is present in vessels and PVAT of the male Sprague Dawley rat aorta (A) and mesenteric vessel (B) as well as in the adipocytes and SVF of the mesenteric PVAT(C). Piezo1, Piezo2, TRPV4, TMEM16, and Panx1 mRNA were measured using RT-PCR and beta actin as a calibrator control. Bars are mean+SEM with scattered individual points for an N = 4–5. Stat test was a one-way ANOVA, *p < 0.05.

Techniques: Reverse Transcription Polymerase Chain Reaction, Control

Detection of Piezo1 in distinct layers (endothelium [E], media [M], and PVAT [P]) of isolated rat thoracic aorta (A), superior mesenteric artery (B), mesenteric resistance vessels (artery [A] and vein [V]) (C), and positive control rat kidney (D). L = lumen. Top panel for each set of images shows DAPI as a nuclei marker and FITC indicating Piezo1 signal, while bottom panel shows tissue without primary antibody (no 1°). White arrows indicate staining of interest. Images are representative of N = 5 taken at 20x with 100 μm scale bars bottom right.

Journal: Pharmacological research

Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

doi: 10.1016/j.phrs.2021.105995

Figure Lengend Snippet: Detection of Piezo1 in distinct layers (endothelium [E], media [M], and PVAT [P]) of isolated rat thoracic aorta (A), superior mesenteric artery (B), mesenteric resistance vessels (artery [A] and vein [V]) (C), and positive control rat kidney (D). L = lumen. Top panel for each set of images shows DAPI as a nuclei marker and FITC indicating Piezo1 signal, while bottom panel shows tissue without primary antibody (no 1°). White arrows indicate staining of interest. Images are representative of N = 5 taken at 20x with 100 μm scale bars bottom right.

Techniques: Isolation, Positive Control, Marker, Staining

Piezo1 agonist Yoda1 induced relaxation of the longitudinal strip of thoracic aorta with endothelium in mouse (A) but not rat (B). (A,B) +E = Endothelium intact (ACh caused >40% PE relaxation), PVAT removed, thoracic aorta in strips. Points shown as mean±SEM for N reported. Star is significance from two-way ANOVA p < 0.05.

Journal: Pharmacological research

Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

doi: 10.1016/j.phrs.2021.105995

Figure Lengend Snippet: Piezo1 agonist Yoda1 induced relaxation of the longitudinal strip of thoracic aorta with endothelium in mouse (A) but not rat (B). (A,B) +E = Endothelium intact (ACh caused >40% PE relaxation), PVAT removed, thoracic aorta in strips. Points shown as mean±SEM for N reported. Star is significance from two-way ANOVA p < 0.05.

Techniques: Stripping Membranes

(A) Isometric tracing for Yoda1 CRC representing (B) quantification of Piezo1 agonist Yoda1 in rat thoracic aorta rings without PVAT, with and without endothelium (+E [78.22 ± 1.63% relaxation to ACh], −E [0.25 ± 0.57% relaxation to ACh], filled, open respectively) and without potassium (−K, circles) and with potassium (+K, triangles). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B) Symbols represent mean±SEM for N reported.

Journal: Pharmacological research

Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

doi: 10.1016/j.phrs.2021.105995

Figure Lengend Snippet: (A) Isometric tracing for Yoda1 CRC representing (B) quantification of Piezo1 agonist Yoda1 in rat thoracic aorta rings without PVAT, with and without endothelium (+E [78.22 ± 1.63% relaxation to ACh], −E [0.25 ± 0.57% relaxation to ACh], filled, open respectively) and without potassium (−K, circles) and with potassium (+K, triangles). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B) Symbols represent mean±SEM for N reported.

Techniques:

Effect of vehicle (DMSO, black) or Piezo1 agonist Yoda1 (grey) on rat superior mesenteric artery contraction (A) or relaxation (B; half-maximally contracted with PE) with or without PVAT (filled or open symbols, respectively). Points represent mean±SEM for N reported.

Journal: Pharmacological research

Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

doi: 10.1016/j.phrs.2021.105995

Figure Lengend Snippet: Effect of vehicle (DMSO, black) or Piezo1 agonist Yoda1 (grey) on rat superior mesenteric artery contraction (A) or relaxation (B; half-maximally contracted with PE) with or without PVAT (filled or open symbols, respectively). Points represent mean±SEM for N reported.

Techniques: